A1 Refereed original research article in a scientific journal
ASSOCIATION OF NUCLEOSIDES AND THEIR 5'-MONOPHOSPHATES WITH A TRYPTOPHAN CONTAINING TRIPEPTIDE, TRP-LEU-GLU - THE SOURCE OF AN OVERESTIMATION BY FLUORESCENCE SPECTROSCOPY
Authors: WIECZOREK Z, STEPINSKI J, DARZYNKIEWICZ E, LONNBERG H
Publisher: ELSEVIER SCIENCE BV
Publication year: 1993
Journal:: Biophysical Chemistry
Journal name in source: BIOPHYSICAL CHEMISTRY
Journal acronym: BIOPHYS CHEM
Volume: 47
Issue: 3
First page : 233
Last page: 240
Number of pages: 8
ISSN: 0301-4622
DOI: https://doi.org/10.1016/0301-4622(93)80048-N
Abstract
Association of 7-methylguanosine 5'-monophosphate with a tryptophan containing tripeptide, Trp-Leu-Glu, has been studied by fluorescence titration using two different geometries of detection, viz. right angle and front surface geometry. The applicability of these two techniques to determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.
Association of 7-methylguanosine 5'-monophosphate with a tryptophan containing tripeptide, Trp-Leu-Glu, has been studied by fluorescence titration using two different geometries of detection, viz. right angle and front surface geometry. The applicability of these two techniques to determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.
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