A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells
Tekijät: Mauretti A, Rossi F, Bax NAM, Miano C, Miraldi F, Goumans MJ, Messina E, Giacomello A, Bouten CVC, Sahlgren C
Kustantaja: CAMBRIDGE UNIV PRESS
Julkaisuvuosi: 2017
Journal: MRS Communications
Tietokannassa oleva lehden nimi: MRS COMMUNICATIONS
Lehden akronyymi: MRS COMMUN
Vuosikerta: 7
Numero: 3
Aloitussivu: 496
Lopetussivu: 501
Sivujen määrä: 6
ISSN: 2159-6859
DOI: https://doi.org/10.1557/mrc.2017.82
Tiivistelmä
Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O-2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.
Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O-2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.