A1 Refereed original research article in a scientific journal
Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells
Authors: Mauretti A, Rossi F, Bax NAM, Miano C, Miraldi F, Goumans MJ, Messina E, Giacomello A, Bouten CVC, Sahlgren C
Publisher: CAMBRIDGE UNIV PRESS
Publication year: 2017
Journal: MRS Communications
Journal name in source: MRS COMMUNICATIONS
Journal acronym: MRS COMMUN
Volume: 7
Issue: 3
First page : 496
Last page: 501
Number of pages: 6
ISSN: 2159-6859
DOI: https://doi.org/10.1557/mrc.2017.82
Abstract
Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O-2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.
Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O-2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.
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