A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Planar chromatographic analysis and quantification of short-lived radioactive metabolites from microdialysis fractions
Tekijät: Haaparanta M, Gronroos T, Eskola O, Bergman J, Solin O
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2006
Journal: Journal of Chromatography A
Tietokannassa oleva lehden nimi: JOURNAL OF CHROMATOGRAPHY A
Lehden akronyymi: J CHROMATOGR A
Vuosikerta: 1108
Numero: 1
Aloitussivu: 136
Lopetussivu: 139
Sivujen määrä: 4
ISSN: 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2005.12.107
Tiivistelmä
A sensitive radiochromatographic method for the quantitative determination of compounds labelled with short-lived beta-emitting radionuclides in microdialysates is described. The method is well suited for microdialysis (MD) samples, which have small volumes and low concentrations of compunds. An F-18-labelled (beta(+); T-1/2 = 109.8 min) radiopharmaceutical, (1R,2S)-4-[F-18]fluorometaraminol (FMR), was injected intravenously into rats, and microdialysis fractions were then collected from the blood at 15 min intervals. Fractions were analyzed for FMR and its radioactive metabolites by planar chromatography combined with digital photostimulated luminescence autoradiography. The lowest detectable F-18-radioactivity was 0.24 Bq/application and the limit of: quantification was 0.31 Bq/application with 4-16 h exposure. The method was found to be highly sensitive and linear in the range of 0.1 Bq-2 kBq. This method thus allows the quantification of beta-emitting radiopharmaceuticals in sequential microdialysis fractions with good time-resolution. (c) 2006 Published by Elsevier B.V.
A sensitive radiochromatographic method for the quantitative determination of compounds labelled with short-lived beta-emitting radionuclides in microdialysates is described. The method is well suited for microdialysis (MD) samples, which have small volumes and low concentrations of compunds. An F-18-labelled (beta(+); T-1/2 = 109.8 min) radiopharmaceutical, (1R,2S)-4-[F-18]fluorometaraminol (FMR), was injected intravenously into rats, and microdialysis fractions were then collected from the blood at 15 min intervals. Fractions were analyzed for FMR and its radioactive metabolites by planar chromatography combined with digital photostimulated luminescence autoradiography. The lowest detectable F-18-radioactivity was 0.24 Bq/application and the limit of: quantification was 0.31 Bq/application with 4-16 h exposure. The method was found to be highly sensitive and linear in the range of 0.1 Bq-2 kBq. This method thus allows the quantification of beta-emitting radiopharmaceuticals in sequential microdialysis fractions with good time-resolution. (c) 2006 Published by Elsevier B.V.
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