A1 Refereed original research article in a scientific journal
Planar chromatographic analysis and quantification of short-lived radioactive metabolites from microdialysis fractions
Authors: Haaparanta M, Gronroos T, Eskola O, Bergman J, Solin O
Publisher: ELSEVIER SCIENCE BV
Publication year: 2006
Journal: Journal of Chromatography A
Journal name in source: JOURNAL OF CHROMATOGRAPHY A
Journal acronym: J CHROMATOGR A
Volume: 1108
Issue: 1
First page : 136
Last page: 139
Number of pages: 4
ISSN: 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2005.12.107
Abstract
A sensitive radiochromatographic method for the quantitative determination of compounds labelled with short-lived beta-emitting radionuclides in microdialysates is described. The method is well suited for microdialysis (MD) samples, which have small volumes and low concentrations of compunds. An F-18-labelled (beta(+); T-1/2 = 109.8 min) radiopharmaceutical, (1R,2S)-4-[F-18]fluorometaraminol (FMR), was injected intravenously into rats, and microdialysis fractions were then collected from the blood at 15 min intervals. Fractions were analyzed for FMR and its radioactive metabolites by planar chromatography combined with digital photostimulated luminescence autoradiography. The lowest detectable F-18-radioactivity was 0.24 Bq/application and the limit of: quantification was 0.31 Bq/application with 4-16 h exposure. The method was found to be highly sensitive and linear in the range of 0.1 Bq-2 kBq. This method thus allows the quantification of beta-emitting radiopharmaceuticals in sequential microdialysis fractions with good time-resolution. (c) 2006 Published by Elsevier B.V.
A sensitive radiochromatographic method for the quantitative determination of compounds labelled with short-lived beta-emitting radionuclides in microdialysates is described. The method is well suited for microdialysis (MD) samples, which have small volumes and low concentrations of compunds. An F-18-labelled (beta(+); T-1/2 = 109.8 min) radiopharmaceutical, (1R,2S)-4-[F-18]fluorometaraminol (FMR), was injected intravenously into rats, and microdialysis fractions were then collected from the blood at 15 min intervals. Fractions were analyzed for FMR and its radioactive metabolites by planar chromatography combined with digital photostimulated luminescence autoradiography. The lowest detectable F-18-radioactivity was 0.24 Bq/application and the limit of: quantification was 0.31 Bq/application with 4-16 h exposure. The method was found to be highly sensitive and linear in the range of 0.1 Bq-2 kBq. This method thus allows the quantification of beta-emitting radiopharmaceuticals in sequential microdialysis fractions with good time-resolution. (c) 2006 Published by Elsevier B.V.
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