A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Microbiome symbionts and diet diversity incur costs on the immune system of insect larvae.
Tekijät: Krams IA, Kecko S, Joers P, Trakimas G, Elferts D, Krams R, Luoto S, Rantala MJ, Inashkina I, Gudra D, Fridmanis D, Contreras-Garduno J, Grantina-Ievina L, Krama T
Kustantaja: COMPANY OF BIOLOGISTS LTD
Julkaisuvuosi: 2017
Journal: Journal of Experimental Biology
Tietokannassa oleva lehden nimi: JOURNAL OF EXPERIMENTAL BIOLOGY
Lehden akronyymi: J EXP BIOL
Vuosikerta: 220
Numero: 22
Aloitussivu: 4204
Lopetussivu: 4212
Sivujen määrä: 9
ISSN: 0022-0949
eISSN: 1477-9145
DOI: https://doi.org/10.1242/jeb.169227
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/27642718
Communities of symbiotic microorganisms that colonize the gastrointestinal tract play an important role in food digestion and protection against opportunistic microbes. Diet diversity increases the number of symbionts in the intestines, a benefit that is considered to impose no cost for the host organism. However, less is known about the possible immunological investments that hosts have to make in order to control the infections caused by symbiont populations that increase because of diet diversity. Using taxonomical composition analysis of the 16S rRNAV3 region, we show that enterococci are the dominating group of bacteria in the midgut of the larvae of the greater wax moth (Galleria mellonella). We found that the number of colony-forming units of enterococci and expressions of certain immunity-related antimicrobial peptide (AMP) genes such as Gallerimycin, Gloverin, 6-tox, Cecropin-D and Galiomicin increased in response to a more diverse diet, which in turn decreased the encapsulation response of the larvae. Treatment with antibiotics significantly lowered the expression of all AMP genes. Diet and antibiotic treatment interaction did not affect the expression of Gloverin and Galiomicin AMP genes, but significantly influenced the expression of Gallerimycin, 6-tox and Cecropin-D. Taken together, our results suggest that diet diversity influences microbiome diversity and AMP gene expression, ultimately affecting an organism's capacity to mount an immune response. Elevated basal levels of immunity-related genes (Gloverin and Galiomicin) might act as a prophylactic against opportunistic infections and as a mechanism that controls the gut symbionts. This would indicate that a diverse diet imposes higher immunity costs on organisms.
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