Selective regulation of Notch ligands during angiogenesis is mediated by vimentin - UTU Research Portal

A1 Refereed original research article in a scientific journal

Selective regulation of Notch ligands during angiogenesis is mediated by vimentin

Authors: Antfolk D, Sjöqvist M, Cheng F, Isoniemi K, Duran CL, Rivero-Müller A, Antila C, Niemi R, Landor S, Bouten CVC, Bayless KJ, Eriksson JE, Sahlgren CM

Publisher: NATL ACAD SCIENCES

Publication year: 2017

Journal: Proceedings of the National Academy of Sciences of the United States of America

Journal name in source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Journal acronym: P NATL ACAD SCI USA

Volume: 114

Issue: 23

First page : E4574

Last page: E4581

Number of pages: 8

ISSN: 0027-8424

eISSN: 1091-6490

DOI: https://doi.org/10.1073/pnas.1703057114

Web address : http://www.pnas.org/content/114/23/E4574

Abstract

Notch signaling is a key regulator of angiogenesis, in which sprouting is regulated by an equilibrium between inhibitory Dll4-Notch signaling and promoting Jagged-Notch signaling. Whereas Fringe proteins modify Notch receptors and strengthen their activation by Dll4 ligands, other mechanisms balancing Jagged and Dll4 signaling are yet to be described. The intermediate filament protein vimentin, which has been previously shown to affect vascular integrity and regenerative signaling, is here shown to regulate ligand-specific Notch signaling. Vimentin interacts with Jagged, impedes basal recycling endocytosis of ligands, but is required for efficient receptor ligand transendocytosis and Notch activation upon receptor binding. Analyses of Notch signal activation by using chimeric ligands with swapped intracellular domains (ICDs), demonstrated that the Jagged ICD binds to vimentin and contributes to signaling strength. Vimentin also suppresses expression of Fringe proteins, whereas depletion of vimentin enhances Fringe levels to promote Dll4 signaling. In line with these data, the vasculature in vimentin knockout (VimKO) embryos and placental tissue is underdeveloped with reduced branching. Disrupted angiogenesis in aortic rings from VimKO mice and in endothelial 3D sprouting assays can be rescued by reactivating Notch signaling by recombinant Jagged ligands. Taken together, we reveal a function of vimentin and demonstrate that vimentin regulates Notch ligand signaling activities during angiogenesis.