A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Targeting of vascular adhesion protein-1 by positron emission tomography visualizes sites of inflammation in Borrelia burgdorferi-infected mice
Tekijät: Riikka Siitonen, Annukka Pietikäinen, Heidi Liljenbäck, Meeri Käkelä, Mirva Söderström, Sirpa Jalkanen, Jukka Hytönen, Anne Roivainen
Kustantaja: BIOMED CENTRAL LTD
Kustannuspaikka: LONDON
Julkaisuvuosi: 2017
Journal: Arthritis Research and Therapy
Tietokannassa oleva lehden nimi: ARTHRITIS RESEARCH & THERAPY
Lehden akronyymi: ARTHRITIS RES THER
Artikkelin numero: 254
Vuosikerta: 19
Sivujen määrä: 10
ISSN: 1478-6354
DOI: https://doi.org/10.1186/s13075-017-1460-4
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/27386389
Background: In the present study, we sought to evaluate the feasibility of targeting vascular adhesion protein-1 (VAP-1) by positron emission tomography (PET) for the longitudinal quantitative assessment of Borrelia burgdorferi infection-induced inflammation in mice.Methods: Mice with B. burgdorferi infection-induced arthritis were studied. During a 7-week follow-up period, the progression of arthritis was monitored weekly with 68Ga-DOTA-Siglec-9 PET/computed tomography (CT) and measurement of tibiotarsal joint swellings. A subgroup of infected mice was treated with ceftriaxone. Finally, histopathological assessment of joint inflammation was performed and VAP-1 expression in joints were determined.Results: Explicit joint swelling and 68Ga-DOTA-Siglec-9 uptake could be demonstrated in the affected joints from B. burgdorferi-infected mice. By contrast, no obvious accumulation of 68Ga-DOTA-Siglec-9 was detected in joints of uninfected mice. The maximum swelling and highest uptake in the affected joints were observed 4 weeks after the infection. 68Ga-DOTA-Siglec-9 uptake in joints correlated with joint swelling (P < 0.0001) and histopathological scoring of inflammation (P = 0.020). Despite short-term antibiotic treatment, the arthritis persisted, and the PET signal remained as high as in nontreated mice. Immunohistochemistry revealed strong-to-moderate expression of VAP-1 in the synovium of B. burgdorferi-infected mice, while only weak expression of VAP-1 was detected in uninfected mice.Conclusions: The present study showed that 68Ga-DOTA-Siglec-9 can detect B. burgdorferi infection-induced arthritis in mice. Furthermore, longitudinal PET/CT imaging allowed monitoring of arthritis development over time.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
