Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase



: Wakadkar S, Zhang LQ, Li DC, Haikarainen T, Dhavala P, Papageorgiou AC

Publisher: WILEY-BLACKWELL PUBLISHING, INC

: 2010

: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS

: ACTA CRYSTALLOGR F

: 66

: 1089

: 1092

: 4

: 1744-3091

DOI: https://doi.org/10.1107/S1744309110030393


Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 A resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P6(1)/P6(5), with unit-cell parameters a = 90.2, c = 314.5 A and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure-activity relationship, assembly and thermal stability of Cu,ZnSODs.



Last updated on 2024-26-11 at 20:09