A1 Refereed original research article in a scientific journal

Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase




AuthorsWakadkar S, Zhang LQ, Li DC, Haikarainen T, Dhavala P, Papageorgiou AC

PublisherWILEY-BLACKWELL PUBLISHING, INC

Publication year2010

JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications

Journal name in sourceACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS

Journal acronymACTA CRYSTALLOGR F

Volume66

First page 1089

Last page1092

Number of pages4

ISSN1744-3091

DOIhttps://doi.org/10.1107/S1744309110030393(external)


Abstract
Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 A resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P6(1)/P6(5), with unit-cell parameters a = 90.2, c = 314.5 A and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure-activity relationship, assembly and thermal stability of Cu,ZnSODs.



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