A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Nestin contributes to skeletal muscle homeostasis and regeneration
Tekijät: Lindqvist J, Torvaldson E, Gullmets J, Karvonen H, Nagy A, Taimen P, Eriksson JE
Kustantaja: COMPANY OF BIOLOGISTS LTD
Julkaisuvuosi: 2017
Journal: Journal of Cell Science
Tietokannassa oleva lehden nimi: JOURNAL OF CELL SCIENCE
Lehden akronyymi: J CELL SCI
Vuosikerta: 130
Numero: 17
Aloitussivu: 2833
Lopetussivu: 2842
Sivujen määrä: 10
ISSN: 0021-9533
DOI: https://doi.org/10.1242/jcs.202226
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/26621223
Nestin, a member of the cytoskeletal family of intermediate filaments, regulates the onset of myogenic differentiation through bidirectional signaling with the kinase Cdk5. Here, we show that these effects are also reflected at the organism level, as there is a loss of skeletal muscle mass in nestin(-/-) (NesKO) mice, reflected as reduced lean (muscle) mass in the mice. Further examination of muscles in male mice revealed that these effects stemmed from nestin- deficient muscles being more prone to spontaneous regeneration. When the regeneration capacity of the compromised NesKO muscle was tested by muscle injury experiments, a significant healing delay was observed. NesKO satellite cells showed delayed proliferation kinetics in conjunction with an elevation in p35 (encoded by Cdk5r1) levels and Cdk5 activity. These results reveal that nestin deficiency generates a spontaneous regenerative phenotype in skeletal muscle that relates to a disturbed proliferation cycle that is associated with uncontrolled Cdk5 activity.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
