A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Analysis of SUMO1-conjugation at synapses
Tekijät: Daniel JA, Cooper BH, Palvimo JJ, Zhang FP, Brose N, Tirard M
Kustantaja: ELIFE SCIENCES PUBLICATIONS LTD
Julkaisuvuosi: 2017
Journal: eLife
Tietokannassa oleva lehden nimi: ELIFE
Lehden akronyymi: ELIFE
Artikkelin numero: ARTN e26338
Vuosikerta: 6
Aloitussivu: 1
Lopetussivu: 36
Sivujen määrä: 36
ISSN: 2050-084X
DOI: https://doi.org/10.7554/eLife.26338
Verkko-osoite: https://research.utu.fi/converis/portal/Publication/26330217
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/26330217
SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in reporter mouse line expressing tagged SUMO1, we could not detect SUMO1-conjugation of seven previously proposed synaptic SUMO1-targets in the brain. Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice showed that anti-SUMO1 immunolabelling at synapses is non-specific. Our findings indicate that SUMO1-conjugation of synaptic proteins does not occur or is extremely rare and hence not detectable using current methodology. Based on our data, we discuss a set of experimental strategies and minimal consensus criteria for the validation of SUMOylation that can be applied to any SUMOylation substrate and SUMO isoform.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
