A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Chromatin organization regulates viral egress dynamics
Tekijät: Aho V, Myllys M, Ruokolainen V, Hakanen S, Mäntylä E, Virtanen J, Hukkanen V, Kühn T, Timonen J, Mattila K, Larabell CA, Vihinen-Ranta M
Kustantaja: NATURE PUBLISHING GROUP
Julkaisuvuosi: 2017
Journal: Scientific Reports
Tietokannassa oleva lehden nimi: SCIENTIFIC REPORTS
Lehden akronyymi: SCI REP-UK
Artikkelin numero: 3692
Vuosikerta: 7
Aloitussivu: 1
Lopetussivu: 10
Sivujen määrä: 10
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-017-03630-y
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/26329411
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
