A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Mechanisms of catalysis by imidazole buffers of the hydrolysis and isomerisation of RNA models
Tekijät: Beckmann C, Kirby AJ, Kuusela S, Tickle DC
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 1998
Journal: Royal Society of Chemistry. Journal: Perkin Transactions 2
Tietokannassa oleva lehden nimi: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Lehden akronyymi: J CHEM SOC PERK T 2
Numero: 3
Aloitussivu: 573
Lopetussivu: 581
Sivujen määrä: 9
ISSN: 0300-9580
DOI: https://doi.org/10.1039/a707741f
We conclude that the hydrolysis of the internucleoside bond in these phosphodiesters involves two, parallel, reaction pathways: (a) a more or less concerted general base catalysed reaction; and (b) a two-step process, involving the rate determining general acid catalysed breakdown of the phosphorane monoanion intermediate 10. Near pH 7, in the presence of Im and ImH(+), the two pathways fun at comparable, but very slow rates. Ribonucleases logically combine the catalytic advantages of both pathways.
