Mechanisms of catalysis by imidazole buffers of the hydrolysis and isomerisation of RNA models - UTU Research Portal

A1 Refereed original research article in a scientific journal

Mechanisms of catalysis by imidazole buffers of the hydrolysis and isomerisation of RNA models

Authors: Beckmann C, Kirby AJ, Kuusela S, Tickle DC

Publisher: ROYAL SOC CHEMISTRY

Publication year: 1998

Journal: Royal Society of Chemistry. Journal: Perkin Transactions 2

Journal name in source: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2

Journal acronym: J CHEM SOC PERK T 2

Issue: 3

First page : 573

Last page: 581

Number of pages: 9

ISSN: 0300-9580

DOI: https://doi.org/10.1039/a707741f

Abstract

We conclude that the hydrolysis of the internucleoside bond in these phosphodiesters involves two, parallel, reaction pathways: (a) a more or less concerted general base catalysed reaction; and (b) a two-step process, involving the rate determining general acid catalysed breakdown of the phosphorane monoanion intermediate 10. Near pH 7, in the presence of Im and ImH(+), the two pathways fun at comparable, but very slow rates. Ribonucleases logically combine the catalytic advantages of both pathways.