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A fluorescence spectroscopic study on the binding of mRNA 5 '-cap-analogs to human translation initiation factor eIF4E: a critical evaluation of the sources of error
Tekijät: Wieczorek Z, Darzynkiewicz E, Lonnberg H
Kustantaja: ELSEVIER SCIENCE SA
Julkaisuvuosi: 1998
Lehti:: Journal of Photochemistry and Photobiology B: Biology
Tietokannassa oleva lehden nimi: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Lehden akronyymi: J PHOTOCH PHOTOBIO B
Vuosikerta: 43
Numero: 2
Aloitussivu: 158
Lopetussivu: 163
Sivujen määrä: 6
ISSN: 1011-1344
DOI: https://doi.org/10.1016/S1011-1344(98)00100-6
Tiivistelmä
Equilibrium constants for the association of human protein translation initiation factor eIF4E with two mRNA 5'-cap analogs, namely 7-methylguanosine 5'-triphosphate and P-1-(7-methylguanosine-5') P-3-(guanosine-5') triphosphate, and with guanosine 5'-monophosphate have been redetermined by the fluorescence quenching method taking the inner filter effect of the cap-analog into account. It has been shown that neglecting the latter correction may lead to either underestimation or overestimation of the association constant obtained by applying the Eadie-Hofstee plot: the reasonably firm binding of 7-methylated cap-analogs becomes underestimated, while the weak binding of nonmethylated nucleotides becomes overestimated. (C) 1998 Elsevier Science S.A. All rights reserved.
Equilibrium constants for the association of human protein translation initiation factor eIF4E with two mRNA 5'-cap analogs, namely 7-methylguanosine 5'-triphosphate and P-1-(7-methylguanosine-5') P-3-(guanosine-5') triphosphate, and with guanosine 5'-monophosphate have been redetermined by the fluorescence quenching method taking the inner filter effect of the cap-analog into account. It has been shown that neglecting the latter correction may lead to either underestimation or overestimation of the association constant obtained by applying the Eadie-Hofstee plot: the reasonably firm binding of 7-methylated cap-analogs becomes underestimated, while the weak binding of nonmethylated nucleotides becomes overestimated. (C) 1998 Elsevier Science S.A. All rights reserved.
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