A1 Refereed original research article in a scientific journal
A fluorescence spectroscopic study on the binding of mRNA 5 '-cap-analogs to human translation initiation factor eIF4E: a critical evaluation of the sources of error
Authors: Wieczorek Z, Darzynkiewicz E, Lonnberg H
Publisher: ELSEVIER SCIENCE SA
Publication year: 1998
Journal:: Journal of Photochemistry and Photobiology B: Biology
Journal name in source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Journal acronym: J PHOTOCH PHOTOBIO B
Volume: 43
Issue: 2
First page : 158
Last page: 163
Number of pages: 6
ISSN: 1011-1344
DOI: https://doi.org/10.1016/S1011-1344(98)00100-6
Abstract
Equilibrium constants for the association of human protein translation initiation factor eIF4E with two mRNA 5'-cap analogs, namely 7-methylguanosine 5'-triphosphate and P-1-(7-methylguanosine-5') P-3-(guanosine-5') triphosphate, and with guanosine 5'-monophosphate have been redetermined by the fluorescence quenching method taking the inner filter effect of the cap-analog into account. It has been shown that neglecting the latter correction may lead to either underestimation or overestimation of the association constant obtained by applying the Eadie-Hofstee plot: the reasonably firm binding of 7-methylated cap-analogs becomes underestimated, while the weak binding of nonmethylated nucleotides becomes overestimated. (C) 1998 Elsevier Science S.A. All rights reserved.
Equilibrium constants for the association of human protein translation initiation factor eIF4E with two mRNA 5'-cap analogs, namely 7-methylguanosine 5'-triphosphate and P-1-(7-methylguanosine-5') P-3-(guanosine-5') triphosphate, and with guanosine 5'-monophosphate have been redetermined by the fluorescence quenching method taking the inner filter effect of the cap-analog into account. It has been shown that neglecting the latter correction may lead to either underestimation or overestimation of the association constant obtained by applying the Eadie-Hofstee plot: the reasonably firm binding of 7-methylated cap-analogs becomes underestimated, while the weak binding of nonmethylated nucleotides becomes overestimated. (C) 1998 Elsevier Science S.A. All rights reserved.
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