A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A human ImmunoChip cDNA microarray provides a comprehensive tool to study immune responses
Tekijät: Nikula T, West A, Katajamaa M, Lonnberg T, Sara R, Aittokallio T, Nevalainen OS, Lahesmaa R
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2005
Tietokannassa oleva lehden nimi: JOURNAL OF IMMUNOLOGICAL METHODS
Lehden akronyymi: J IMMUNOL METHODS
Vuosikerta: 303
Numero: 1-2
Aloitussivu: 122
Lopetussivu: 134
Sivujen määrä: 13
ISSN: 0022-1759
DOI: https://doi.org/10.1016/j.jim.2005.06.004
Tiivistelmä
DNA microarray technology has developed rapidly in recent years and has become an essential tool, providing novel approaches to biomedical research. In this paper, we describe a self-designed ImmunoChip cDNA array for immunological research. With a comprehensive selection of genes of interest, we can focus on key signalling pathways and molecular mechanisms at relatively low cost compared to commercial platforms which are usually targeted at global screening of gene expression. To validate the efficiency of the ImmunoChip, we studied T helper cell polarization to functionally distinct subsets (Th1 and Th2). We also developed a tool for quality control of cDNA microarrays that assesses the technical quality of an ImmunoChip. The information produced with the quality control tool is shown to be valuable for extracting correct information from cDNA microarrays. Gene expression measurements with ImmunoChip are in agreement with the results obtained using oligonucleotide microarrays and with published quantitative RT-PCR data. The ImmunoChip provides reliable measurements and gives new insights into various aspects of human immune responses. (c) 2005 Elsevier B.V. All rights reserved.
DNA microarray technology has developed rapidly in recent years and has become an essential tool, providing novel approaches to biomedical research. In this paper, we describe a self-designed ImmunoChip cDNA array for immunological research. With a comprehensive selection of genes of interest, we can focus on key signalling pathways and molecular mechanisms at relatively low cost compared to commercial platforms which are usually targeted at global screening of gene expression. To validate the efficiency of the ImmunoChip, we studied T helper cell polarization to functionally distinct subsets (Th1 and Th2). We also developed a tool for quality control of cDNA microarrays that assesses the technical quality of an ImmunoChip. The information produced with the quality control tool is shown to be valuable for extracting correct information from cDNA microarrays. Gene expression measurements with ImmunoChip are in agreement with the results obtained using oligonucleotide microarrays and with published quantitative RT-PCR data. The ImmunoChip provides reliable measurements and gives new insights into various aspects of human immune responses. (c) 2005 Elsevier B.V. All rights reserved.
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