A1 Refereed original research article in a scientific journal
A human ImmunoChip cDNA microarray provides a comprehensive tool to study immune responses
Authors: Nikula T, West A, Katajamaa M, Lonnberg T, Sara R, Aittokallio T, Nevalainen OS, Lahesmaa R
Publisher: ELSEVIER SCIENCE BV
Publication year: 2005
Journal name in source: JOURNAL OF IMMUNOLOGICAL METHODS
Journal acronym: J IMMUNOL METHODS
Volume: 303
Issue: 1-2
First page : 122
Last page: 134
Number of pages: 13
ISSN: 0022-1759
DOI: https://doi.org/10.1016/j.jim.2005.06.004
Abstract
DNA microarray technology has developed rapidly in recent years and has become an essential tool, providing novel approaches to biomedical research. In this paper, we describe a self-designed ImmunoChip cDNA array for immunological research. With a comprehensive selection of genes of interest, we can focus on key signalling pathways and molecular mechanisms at relatively low cost compared to commercial platforms which are usually targeted at global screening of gene expression. To validate the efficiency of the ImmunoChip, we studied T helper cell polarization to functionally distinct subsets (Th1 and Th2). We also developed a tool for quality control of cDNA microarrays that assesses the technical quality of an ImmunoChip. The information produced with the quality control tool is shown to be valuable for extracting correct information from cDNA microarrays. Gene expression measurements with ImmunoChip are in agreement with the results obtained using oligonucleotide microarrays and with published quantitative RT-PCR data. The ImmunoChip provides reliable measurements and gives new insights into various aspects of human immune responses. (c) 2005 Elsevier B.V. All rights reserved.
DNA microarray technology has developed rapidly in recent years and has become an essential tool, providing novel approaches to biomedical research. In this paper, we describe a self-designed ImmunoChip cDNA array for immunological research. With a comprehensive selection of genes of interest, we can focus on key signalling pathways and molecular mechanisms at relatively low cost compared to commercial platforms which are usually targeted at global screening of gene expression. To validate the efficiency of the ImmunoChip, we studied T helper cell polarization to functionally distinct subsets (Th1 and Th2). We also developed a tool for quality control of cDNA microarrays that assesses the technical quality of an ImmunoChip. The information produced with the quality control tool is shown to be valuable for extracting correct information from cDNA microarrays. Gene expression measurements with ImmunoChip are in agreement with the results obtained using oligonucleotide microarrays and with published quantitative RT-PCR data. The ImmunoChip provides reliable measurements and gives new insights into various aspects of human immune responses. (c) 2005 Elsevier B.V. All rights reserved.
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