A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Susceptibility of Fusobacterium nucleatum to killing by peroxidase-iodide-hydrogen peroxide combination in buffer solution and in human whole saliva
Tekijät: Ihalin R, Nuutila J, Loimaranta V, Lenander M, Tenovuo J, Lilius EM
Kustantaja: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
Julkaisuvuosi: 2003
Journal: Anaerobe
Tietokannassa oleva lehden nimi: ANAEROBE
Lehden akronyymi: ANAEROBE
Vuosikerta: 9
Numero: 1
Aloitussivu: 23
Lopetussivu: 30
Sivujen määrä: 8
ISSN: 1075-9964
DOI: https://doi.org/10.1016/S1075-9964(03)00005-2
Verkko-osoite: https://www.sciencedirect.com/science/article/pii/S1075996403000052?via=ihub
Tiivistelmä
Some Gram-negative anaerobic bacteria have been associated with the infection of tooth supporting tissues, i.e. periodontitis. Of these bacteria, Fusobacterium nucleation is sensitive to lactoperoxidase/myeloperoxidase-iodide-hydrogen peroxide system in vitro, but salivary concentrations of thiocyanate abolishes the bactericidality. These bacteria are located in periodontal pockets, on oral mucosa and in saliva. Although F nucleatum most probably does not belong to the group of main periodontal pathogens, it sustains its proportion in the periodontal flora when gingivitis progresses to periodontitis. In this study, the sensitivity of F nucleatum to different horseradish peroxidase-iodide-hydrogen peroxide combinations was tested both in buffer and in sterilized human whole saliva. Horseradish peroxidase was chosen because it does not bind thiocyanate at pH greater than or equal to 6. After I h incubation at 37degreesC, the cell viability was estimated by plate count and with flow cytometer using LIVE/DEAD((R)) BacLight(TM) kit (Molecular Probes, USA). In saliva. the horseradish peroxidase (50 mug/mL)-iodide (2.5 mM)-hydrogen peroxide (2.5 mM) combination decreased the amount of viable bacteria to 37% compared to 85% in the control without any of the components when measured with flow cytometer. Replacement of buffer by saliva decreased the bactericidality of the peroxidase system. However, in buffer less iodide and hydrogen peroxide was needed to produce significant decrease in the number of viable bacteria when measured by plate count than with flow cytometer. Our study shows that horseradish peroxidase-iodide-hydrogen peroxide combination is able to kill F nucleatum cells in saliva. Horseradish peroxidase-iodide-hydrogen peroxide combination may be useful to diminish the degree of re-colonization of periodontitis-associated bacteria after periodontal therapy and to inhibit the transmission of these bacteria via saliva. (C) 2003 Elsevier Science Ltd. All rights reserved.
Some Gram-negative anaerobic bacteria have been associated with the infection of tooth supporting tissues, i.e. periodontitis. Of these bacteria, Fusobacterium nucleation is sensitive to lactoperoxidase/myeloperoxidase-iodide-hydrogen peroxide system in vitro, but salivary concentrations of thiocyanate abolishes the bactericidality. These bacteria are located in periodontal pockets, on oral mucosa and in saliva. Although F nucleatum most probably does not belong to the group of main periodontal pathogens, it sustains its proportion in the periodontal flora when gingivitis progresses to periodontitis. In this study, the sensitivity of F nucleatum to different horseradish peroxidase-iodide-hydrogen peroxide combinations was tested both in buffer and in sterilized human whole saliva. Horseradish peroxidase was chosen because it does not bind thiocyanate at pH greater than or equal to 6. After I h incubation at 37degreesC, the cell viability was estimated by plate count and with flow cytometer using LIVE/DEAD((R)) BacLight(TM) kit (Molecular Probes, USA). In saliva. the horseradish peroxidase (50 mug/mL)-iodide (2.5 mM)-hydrogen peroxide (2.5 mM) combination decreased the amount of viable bacteria to 37% compared to 85% in the control without any of the components when measured with flow cytometer. Replacement of buffer by saliva decreased the bactericidality of the peroxidase system. However, in buffer less iodide and hydrogen peroxide was needed to produce significant decrease in the number of viable bacteria when measured by plate count than with flow cytometer. Our study shows that horseradish peroxidase-iodide-hydrogen peroxide combination is able to kill F nucleatum cells in saliva. Horseradish peroxidase-iodide-hydrogen peroxide combination may be useful to diminish the degree of re-colonization of periodontitis-associated bacteria after periodontal therapy and to inhibit the transmission of these bacteria via saliva. (C) 2003 Elsevier Science Ltd. All rights reserved.
Turun yliopiston Tutkimustietojärjestelmän portaali