Covalent immobilization of oligonucleotides to microparticles. Quantitation of hybridization by time-resolved fluorescence detection on a single particle



: Hakala H, Heinonen P, Iitia A, Lonnberg H

Publisher: INST ORGANIC CHEM AND BIOCHEM

: 1996

 Collection of Czechoslovak Chemical Communications

: COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS

: COLLECT CZECH CHEM C

: 61

: S107

: S109

: 3

: 0010-0765


A mixture of microscopic particles, each bearing a given allele-specific oligonucleotide probe and a reporter group defining the particle category, enables simultaneous detection of several gene mutations, providing that each particle may be separately subjected to measurement. It has been shown(1) that a highly sensitive miniaturized assay format may be developed by using uniformly sized (50 mu m) polymethacrylate particles as a solid phase, and a photoluminescent europium chelate for detection. We now report on alternative ways of tethering the oligonucleotide probe to the microparticles, and discuss the effects of various linkers on the hybridization properties.



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