B1 Vertaisarvioimaton kirjoitus tieteellisessä lehdessä
Covalent immobilization of oligonucleotides to microparticles. Quantitation of hybridization by time-resolved fluorescence detection on a single particle
Tekijät: Hakala H, Heinonen P, Iitia A, Lonnberg H
Kustantaja: INST ORGANIC CHEM AND BIOCHEM
Julkaisuvuosi: 1996
Lehti: Collection of Czechoslovak Chemical Communications
Tietokannassa oleva lehden nimi: COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS
Lehden akronyymi: COLLECT CZECH CHEM C
Vuosikerta: 61
Aloitussivu: S107
Lopetussivu: S109
Sivujen määrä: 3
ISSN: 0010-0765
Tiivistelmä
A mixture of microscopic particles, each bearing a given allele-specific oligonucleotide probe and a reporter group defining the particle category, enables simultaneous detection of several gene mutations, providing that each particle may be separately subjected to measurement. It has been shown(1) that a highly sensitive miniaturized assay format may be developed by using uniformly sized (50 mu m) polymethacrylate particles as a solid phase, and a photoluminescent europium chelate for detection. We now report on alternative ways of tethering the oligonucleotide probe to the microparticles, and discuss the effects of various linkers on the hybridization properties.
A mixture of microscopic particles, each bearing a given allele-specific oligonucleotide probe and a reporter group defining the particle category, enables simultaneous detection of several gene mutations, providing that each particle may be separately subjected to measurement. It has been shown(1) that a highly sensitive miniaturized assay format may be developed by using uniformly sized (50 mu m) polymethacrylate particles as a solid phase, and a photoluminescent europium chelate for detection. We now report on alternative ways of tethering the oligonucleotide probe to the microparticles, and discuss the effects of various linkers on the hybridization properties.
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