A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Primers for sequence characterization and polymorphism detection in the Atlantic salmon (Salmo salar) growth hormone 1 (GH1) gene
Tekijät: Ryynanen HJ, Primmer CR
Kustantaja: BLACKWELL PUBLISHING LTD
Julkaisuvuosi: 2004
Lehti: Molecular Ecology Notes
Tietokannassa oleva lehden nimi: MOLECULAR ECOLOGY NOTES
Lehden akronyymi: MOL ECOL NOTES
Vuosikerta: 4
Numero: 4
Aloitussivu: 664
Lopetussivu: 667
Sivujen määrä: 4
ISSN: 1471-8278
DOI: https://doi.org/10.1111/j.1471-8286.2004.00776.x
Tiivistelmä
We report the identification of intraspecific sequence variation in the Atlantic salmon (Salmo salar) growth hormone 1 gene. Rapid and inexpensive assays for polymorphism detection were developed for 10 sites. Five of the assays detected single nucleotide polymorphisms (SNPs) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses, and five were indel polymorphisms, detected using fragment length analyses. The average within population frequency of the most common allele varied from 0.52 to 0.90, and the average within population heterozygosity varied from 0.02 to 0.37 in seven European salmon populations.
We report the identification of intraspecific sequence variation in the Atlantic salmon (Salmo salar) growth hormone 1 gene. Rapid and inexpensive assays for polymorphism detection were developed for 10 sites. Five of the assays detected single nucleotide polymorphisms (SNPs) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses, and five were indel polymorphisms, detected using fragment length analyses. The average within population frequency of the most common allele varied from 0.52 to 0.90, and the average within population heterozygosity varied from 0.02 to 0.37 in seven European salmon populations.
Turun yliopiston Tutkimustietojärjestelmän portaali