A1 Refereed original research article in a scientific journal
Primers for sequence characterization and polymorphism detection in the Atlantic salmon (Salmo salar) growth hormone 1 (GH1) gene
Authors: Ryynanen HJ, Primmer CR
Publisher: BLACKWELL PUBLISHING LTD
Publication year: 2004
Journal: Molecular Ecology Notes
Journal name in source: MOLECULAR ECOLOGY NOTES
Journal acronym: MOL ECOL NOTES
Volume: 4
Issue: 4
First page : 664
Last page: 667
Number of pages: 4
ISSN: 1471-8278
DOI: https://doi.org/10.1111/j.1471-8286.2004.00776.x
Abstract
We report the identification of intraspecific sequence variation in the Atlantic salmon (Salmo salar) growth hormone 1 gene. Rapid and inexpensive assays for polymorphism detection were developed for 10 sites. Five of the assays detected single nucleotide polymorphisms (SNPs) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses, and five were indel polymorphisms, detected using fragment length analyses. The average within population frequency of the most common allele varied from 0.52 to 0.90, and the average within population heterozygosity varied from 0.02 to 0.37 in seven European salmon populations.
We report the identification of intraspecific sequence variation in the Atlantic salmon (Salmo salar) growth hormone 1 gene. Rapid and inexpensive assays for polymorphism detection were developed for 10 sites. Five of the assays detected single nucleotide polymorphisms (SNPs) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses, and five were indel polymorphisms, detected using fragment length analyses. The average within population frequency of the most common allele varied from 0.52 to 0.90, and the average within population heterozygosity varied from 0.02 to 0.37 in seven European salmon populations.
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