A1 Refereed original research article in a scientific journal
Sensitive bioaffinity assays with individual microparticles and time-resolved fluorometry
Authors: Lovgren T, Heinonen P, Lehtinen P, Hakala H, Heinola J, Harju R, Takalo H, Mukkala VM, Schmid R, Lonnberg H, Pettersson K, Iitia A
Publisher: AMER ASSOC CLINICAL CHEMISTRY
Publication year: 1997
Journal:: Clinical Chemistry
Journal name in source: CLINICAL CHEMISTRY
Journal acronym: CLIN CHEM
Volume: 43
Issue: 10
First page : 1937
Last page: 1943
Number of pages: 7
ISSN: 0009-9147
Abstract
Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial reso lution. By using fluorescent europium chelates and time-resolved microfluorometry, one can detect 11 000 europium molecules on individual microparticles. In a miniaturized noncompetitive immunoassay of prostate-specific antigen (PSA), we quantitatively detected 5 ng/L (0.05 amol per particle) of the analyte on an individual microparticle with excellent precision over the whole measurement range (CV <10%). Using a hybridization assay, we also could detect the Delta F508 mutation for cystic fibrosis on individual microparticles. Consequently, fluorescent lanthanide chelate labels and time-resolved microfluorometry qualify as the next generation of technology in this field.
Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial reso lution. By using fluorescent europium chelates and time-resolved microfluorometry, one can detect 11 000 europium molecules on individual microparticles. In a miniaturized noncompetitive immunoassay of prostate-specific antigen (PSA), we quantitatively detected 5 ng/L (0.05 amol per particle) of the analyte on an individual microparticle with excellent precision over the whole measurement range (CV <10%). Using a hybridization assay, we also could detect the Delta F508 mutation for cystic fibrosis on individual microparticles. Consequently, fluorescent lanthanide chelate labels and time-resolved microfluorometry qualify as the next generation of technology in this field.
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