A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Sensitive bioaffinity assays with individual microparticles and time-resolved fluorometry
Tekijät: Lovgren T, Heinonen P, Lehtinen P, Hakala H, Heinola J, Harju R, Takalo H, Mukkala VM, Schmid R, Lonnberg H, Pettersson K, Iitia A
Kustantaja: AMER ASSOC CLINICAL CHEMISTRY
Julkaisuvuosi: 1997
Lehti: Clinical Chemistry
Tietokannassa oleva lehden nimi: CLINICAL CHEMISTRY
Lehden akronyymi: CLIN CHEM
Vuosikerta: 43
Numero: 10
Aloitussivu: 1937
Lopetussivu: 1943
Sivujen määrä: 7
ISSN: 0009-9147
Tiivistelmä
Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial reso lution. By using fluorescent europium chelates and time-resolved microfluorometry, one can detect 11 000 europium molecules on individual microparticles. In a miniaturized noncompetitive immunoassay of prostate-specific antigen (PSA), we quantitatively detected 5 ng/L (0.05 amol per particle) of the analyte on an individual microparticle with excellent precision over the whole measurement range (CV <10%). Using a hybridization assay, we also could detect the Delta F508 mutation for cystic fibrosis on individual microparticles. Consequently, fluorescent lanthanide chelate labels and time-resolved microfluorometry qualify as the next generation of technology in this field.
Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial reso lution. By using fluorescent europium chelates and time-resolved microfluorometry, one can detect 11 000 europium molecules on individual microparticles. In a miniaturized noncompetitive immunoassay of prostate-specific antigen (PSA), we quantitatively detected 5 ng/L (0.05 amol per particle) of the analyte on an individual microparticle with excellent precision over the whole measurement range (CV <10%). Using a hybridization assay, we also could detect the Delta F508 mutation for cystic fibrosis on individual microparticles. Consequently, fluorescent lanthanide chelate labels and time-resolved microfluorometry qualify as the next generation of technology in this field.
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