A1 Refereed original research article in a scientific journal
Large Variability of Proanthocyanidin Content and Composition in Sainfoin (Onobrychis viciifolia)
Authors: Carsten S. Malisch, Andreas Lüscher, Nicolas Baert, Marica T. Engström, Bruno Studer, Christos Fryganas, Daniel Suter, Irene Mueller-Harvey, Juha-Pekka Salminen
Publication year: 2015
Journal: Journal of Agricultural and Food Chemistry
Volume: 63
Issue: 47
First page : 10234
Last page: 10242
Number of pages: 9
ISSN: 0021-8561
DOI: https://doi.org/10.1021/acs.jafc.5b04946
Web address : http://pubs.acs.org/doi/10.1021/acs.jafc.5b04946
Proanthocyanidins (PAs) in sainfoin (Onobrychis viciifolia Scop.) are of interest to ameliorate the sustainability of livestock production. However, sainfoin forage yield and PA concentrations, as well as their composition, require optimization. Individual plants of 27 sainfoin accessions from four continents were analyzed with LC-ESI-QqQ-MS/MS for PA concentrations and simple phenolic compounds. Large variability existed in PA concentrations (23.0−47.5 mg g−1 leaf dry matter (DM)), share of prodelphinidins (79−96%), and mean degree of polymerization (11−14) among, but also within, accessions. PAs were mainly located in leaves (26.8 mg g−1 DM), whereas stems had less PAs (7.8 mg g−1 DM). Overall, high-yielding plants had lower PA leaf concentrations (R2 = 0.16, P < 0.001) and fewer leaves (R2 = 0.66, P < 0.001). However, the results show that these two trade-offs between yield and bioactive PAs can be overcome. KEYWORDS:
