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Fast Genomic Biomarker Responses of Retene and Pyrene in Liver of Juvenile Rainbow Trout, Oncorhynchus mykiss
Tekijät: Räsänen Kati, Arsiola Tiina, Oikari Aimo
Kustantaja: Springer
Julkaisuvuosi: 2012
Journal: Bulletin of Environmental Contamination and Toxicology
Tietokannassa oleva lehden nimi: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Lehden akronyymi: B ENVIRON CONTAM TOX
Numero sarjassa: 4
Vuosikerta: 89
Numero: 4
Aloitussivu: 733
Lopetussivu: 738
Sivujen määrä: 6
ISSN: 0007-4861
DOI: https://doi.org/10.1007/s00128-012-0770-0
Tiivistelmä
We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9-10 mu g/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.
We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9-10 mu g/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.
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