A1 Refereed original research article in a scientific journal
Fast Genomic Biomarker Responses of Retene and Pyrene in Liver of Juvenile Rainbow Trout, Oncorhynchus mykiss
Authors: Räsänen Kati, Arsiola Tiina, Oikari Aimo
Publisher: Springer
Publication year: 2012
Journal: Bulletin of Environmental Contamination and Toxicology
Journal name in source: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Journal acronym: B ENVIRON CONTAM TOX
Number in series: 4
Volume: 89
Issue: 4
First page : 733
Last page: 738
Number of pages: 6
ISSN: 0007-4861
DOI: https://doi.org/10.1007/s00128-012-0770-0
Abstract
We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9-10 mu g/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.
We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9-10 mu g/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.
UTU Research Portal