Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains
: Antal TK, Kukarskikh GP, Bulychev AA, Tyystjärvi E, Krendeleva T
Publisher: SPRINGER
: 2013
: Planta
: PLANTA
: PLANTA
: 5
: 237
: 5
: 1241
: 1250
: 10
: 0032-0935
DOI: https://doi.org/10.1007/s00425-013-1843-y(external)
The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P-700 (+) in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.