A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains
Tekijät: Antal TK, Kukarskikh GP, Bulychev AA, Tyystjärvi E, Krendeleva T
Kustantaja: SPRINGER
Julkaisuvuosi: 2013
Journal: Planta
Tietokannassa oleva lehden nimi: PLANTA
Lehden akronyymi: PLANTA
Numero sarjassa: 5
Vuosikerta: 237
Numero: 5
Aloitussivu: 1241
Lopetussivu: 1250
Sivujen määrä: 10
ISSN: 0032-0935
DOI: https://doi.org/10.1007/s00425-013-1843-y
Tiivistelmä
The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P-700 (+) in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.
The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P-700 (+) in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.
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