A1 Refereed original research article in a scientific journal
Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
Authors: Petri Ihalainen, Himadri Majumdar, Tapani Viitala, Björn Törngren, Tuomas Näreoja, Anni Määttänen, Jawad Sarfraz, Harri Härmä, Marjo Yliperttula, Ronald Österbacka, Jouko Peltonen
Publisher: MDPI AG (Basel, Switzerland)
Publishing place: (Basel, Switzerland)
Publication year: 2013
Journal: Biosensors
Journal acronym: Biosensors
Article number: 1-17
Number in series: 1
Volume: 3
Issue: 1
First page : 1
Last page: 17
ISSN: 2079-6374
DOI: https://doi.org/10.3390/bios3010001
Abstract
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
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