A1 Refereed original research article in a scientific journal
Manipulation of Single-Stranded DNA by Using an Artificial Site-Selective DNA Cutter Composed of Cerium(IV)/EDTA and Phosphonate-Oligonucleotide Conjugates
Authors: Aiba Y, Lonnberg T, Komiyama M
Publisher: WILEY-BLACKWELL
Publication year: 2011
Journal: Chemistry - An Asian Journal
Journal name in source: CHEMISTRY-AN ASIAN JOURNAL
Journal acronym: CHEM-ASIAN J
Number in series: 9
Volume: 6
Issue: 9
First page : 2407
Last page: 2411
Number of pages: 5
ISSN: 1861-4728
DOI: https://doi.org/10.1002/asia.201100238
Self-archived copy’s web address: https://research.utu.fi/converis/portal/Publication/1983568
An artificial site-selective DNA cutter to hydrolyze single-stranded DNA at a desired site was prepared from Ce(IV)/ethylenediamintetraacetic acid (EDTA) and two ethylenediamine-N,N,N',N'-tetrakis(methylenephosphonic acid)-oligonucleotide conjugates. By using this cutter, the sense strand of a blue fluorescent protein (BFP) gene was selectively cut at a predetermined site in the chromophore-coding region. The upstream fragment obtained by the site-selective scission was ligated with the downstream fragment of the closely related green fluorescent protein (GFP) gene so that the 5'- and 3'-end portions of the chromophore came from the BFP fragment and the GFP fragment, respectively. The recombinant gene was successfully expressed in E. coli and the chimeric chromophore emitted green fluorescence as expected.
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