A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

High-performance liquid chromatographic analysis of phlorotannins from the brown alga Fucus vesiculosus




TekijätKoivikko R, Loponen J, Pihlaja K, Jormalainen V

KustantajaJOHN WILEY & SONS LTD

Julkaisuvuosi2007

JournalPhytochemical Analysis

Tietokannassa oleva lehden nimiPHYTOCHEMICAL ANALYSIS

Lehden akronyymiPHYTOCHEM ANALYSIS

Vuosikerta18

Numero4

Aloitussivu326

Lopetussivu332

Sivujen määrä7

ISSN0958-0344

DOIhttps://doi.org/10.1002/pea.986


Tiivistelmä
Separating individual compounds by HPLC represents an effective method for the detection and quantification of phenolic compounds and has been widely utilised. However, phlorotannins are commonly quantified using colorimetric methods, as the total amount of the whole compound group. In the present paper the separation of a set of individual soluble phlorotannins from the phenolic crude extract of Fucus vesiculosus was achieved by HPLC with UV photodiode array detection. Different gradient programs for reversed- and normal-phase HPLC methods were developed and tested. Normal-phase (NP) conditions with a silica stationary phase and a mobile phase with a linear gradient of increasing polarity were found to separate 16 individual components of the phenolic extract. The suitability of the NP-HPLC method for mass spectrometric application was preliminarily tested. Sample preparation was found to be a critical step in the analysis owing to the rapid oxidation of phlorotannins; ascorbic acid was used as an antioxidant. Copyright (C) 2007 John Wiley & Sons, Ltd.



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