A1 Refereed original research article in a scientific journal
High-performance liquid chromatographic analysis of phlorotannins from the brown alga Fucus vesiculosus
Authors: Koivikko R, Loponen J, Pihlaja K, Jormalainen V
Publisher: JOHN WILEY & SONS LTD
Publication year: 2007
Journal: Phytochemical Analysis
Journal name in source: PHYTOCHEMICAL ANALYSIS
Journal acronym: PHYTOCHEM ANALYSIS
Volume: 18
Issue: 4
First page : 326
Last page: 332
Number of pages: 7
ISSN: 0958-0344
DOI: https://doi.org/10.1002/pea.986(external)
Abstract
Separating individual compounds by HPLC represents an effective method for the detection and quantification of phenolic compounds and has been widely utilised. However, phlorotannins are commonly quantified using colorimetric methods, as the total amount of the whole compound group. In the present paper the separation of a set of individual soluble phlorotannins from the phenolic crude extract of Fucus vesiculosus was achieved by HPLC with UV photodiode array detection. Different gradient programs for reversed- and normal-phase HPLC methods were developed and tested. Normal-phase (NP) conditions with a silica stationary phase and a mobile phase with a linear gradient of increasing polarity were found to separate 16 individual components of the phenolic extract. The suitability of the NP-HPLC method for mass spectrometric application was preliminarily tested. Sample preparation was found to be a critical step in the analysis owing to the rapid oxidation of phlorotannins; ascorbic acid was used as an antioxidant. Copyright (C) 2007 John Wiley & Sons, Ltd.
Separating individual compounds by HPLC represents an effective method for the detection and quantification of phenolic compounds and has been widely utilised. However, phlorotannins are commonly quantified using colorimetric methods, as the total amount of the whole compound group. In the present paper the separation of a set of individual soluble phlorotannins from the phenolic crude extract of Fucus vesiculosus was achieved by HPLC with UV photodiode array detection. Different gradient programs for reversed- and normal-phase HPLC methods were developed and tested. Normal-phase (NP) conditions with a silica stationary phase and a mobile phase with a linear gradient of increasing polarity were found to separate 16 individual components of the phenolic extract. The suitability of the NP-HPLC method for mass spectrometric application was preliminarily tested. Sample preparation was found to be a critical step in the analysis owing to the rapid oxidation of phlorotannins; ascorbic acid was used as an antioxidant. Copyright (C) 2007 John Wiley & Sons, Ltd.
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