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Bacteriophage phi YeO3-12, specific for Yersinia enterocolitica serotype O : 3, is related to coliphages T3 and T7
Tekijät: Pajunen M, Kiljunen S, Skurnik M
Kustantaja: AMER SOC MICROBIOLOGY
Julkaisuvuosi: 2000
Lehti:: Journal of Bacteriology
Tietokannassa oleva lehden nimi: JOURNAL OF BACTERIOLOGY
Lehden akronyymi: J BACTERIOL
Vuosikerta: 182
Numero: 18
Aloitussivu: 5114
Lopetussivu: 5120
Sivujen määrä: 7
ISSN: 0021-9193
DOI: https://doi.org/10.1128/JB.182.18.5114-5120.2000
Verkko-osoite: http://jb.asm.org/content/182/18/5114
Tiivistelmä
Bacteriophage phi YeO3-12 is a lytic phage of Yersinia enterocolitica serotype O:3, The phage receptor is the lipopolysaccharide O chain of this serotype that consists of the rare sugar 6-deoxy-L-altropyranose. A one-step growth curve of phi YeO3-12 revealed eclipse and latent periods of 15 and 25 min, respectively, with a burst size of about 120 PFU per infected cell. In electron microscopy phi YeO3-12 virions showed pentagonal outlines, indicating their icosahedral nature. The phage capsid was shown to be composed of at least 10 structural proteins, of which a protein of 43 kDa was predominant. N-terminal sequences of three structural proteins were determined, two of them showing strong homology to structural proteins of coliphages T3 and T7, The phage genome was found to consist of a double-stranded DNA molecule of 40 kb without cohesive ends. A physical map of the phage DNA was constructed using five restriction enzymes, The phage infection could be effectively neutralized using serum from a rabbit immunized with whole phi YeO3-12 particles. The antiserum also neutralized T3 infection, although not as efficiently as that of phi YeO3-12. phi YeO3-12 was found to share, in addition to the N-terminal sequence homology, several common features with T3, including morphology and nonsubjectibility to F exclusion. The evidence conclusively indicated that phi YeO3-12 is the first close relative of phage T3 to be described.
Bacteriophage phi YeO3-12 is a lytic phage of Yersinia enterocolitica serotype O:3, The phage receptor is the lipopolysaccharide O chain of this serotype that consists of the rare sugar 6-deoxy-L-altropyranose. A one-step growth curve of phi YeO3-12 revealed eclipse and latent periods of 15 and 25 min, respectively, with a burst size of about 120 PFU per infected cell. In electron microscopy phi YeO3-12 virions showed pentagonal outlines, indicating their icosahedral nature. The phage capsid was shown to be composed of at least 10 structural proteins, of which a protein of 43 kDa was predominant. N-terminal sequences of three structural proteins were determined, two of them showing strong homology to structural proteins of coliphages T3 and T7, The phage genome was found to consist of a double-stranded DNA molecule of 40 kb without cohesive ends. A physical map of the phage DNA was constructed using five restriction enzymes, The phage infection could be effectively neutralized using serum from a rabbit immunized with whole phi YeO3-12 particles. The antiserum also neutralized T3 infection, although not as efficiently as that of phi YeO3-12. phi YeO3-12 was found to share, in addition to the N-terminal sequence homology, several common features with T3, including morphology and nonsubjectibility to F exclusion. The evidence conclusively indicated that phi YeO3-12 is the first close relative of phage T3 to be described.
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