A3 Refereed book chapter or chapter in a compilation book
Mass Spectrometry-based Serum Proteomics for Biomarker discovery and validation
Authors: Santosh D. Bhosale, Robert Moulder, Petri Kouvonen, Riitta Lahesmaa, David R. Goodlett
Editors: David W. Greening, Richard J. Simpson
Publication year: 2017
Book title : Serum/plasma proteomics : Methods and Protocols
Series title: Serum/Plasma Proteomics
Volume: 1619
First page : 451
Last page: 466
Number of pages: 16
ISBN: 978-1-4939-7056-8
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-4939-7057-5_31
Web address : https://link.springer.com/protocol/10.1007/978-1-4939-7057-5_31
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/19257165
Blood protein measurements are used frequently
in the clinic in the assessment of patient health. Nevertheless, there remains
the need for new biomarkers with better diagnostic specificities. With the
advent of improved technology for bioanalysis and the growth of biobanks including
collections from specific disease-risk cohorts, the plasma proteome has
remained a target of proteomics research towards the characterization of
disease related biomarkers. The following protocol presents a workflow for serum/plasma
proteomics including details of sample preparation both with and without immunoaffinity
depletion of the most abundant plasma proteins, and methodology for selected
reaction monitoring mass spectrometry validation.
Downloadable publication This is an electronic reprint of the original article. |  |
