Cell proliferation and chromosomal changes in human ameloblastoma

: Jaaskelainen K, Jee KJ, Leivo I, Saloniemi I, Knuutila S, Heikinheimo K

Publisher: ELSEVIER SCIENCE INC

: 2002

: Cancer Genetics and Cytogenetics

: CANCER GENETICS AND CYTOGENETICS

: CANCER GENET CYTOGEN

: 136

: 1

: 31

: 37

: 7

: 0165-4608

DOI: https://doi.org/10.1016/S0165-4608(02)00512-5

Cell proliferation and chromosomal imbalances, important parameters in relation to tumor progression, were studied in ameloblastoma (n=20), a benign odontogenic tumor of locally recurrent nature. Immunocytochemical staining with MIB-1 antibody and comparative genomic hybridization (CGH) were performed on formalin-fixed paraffin-embedded ameloblastomas. The mean follow-up time was 12.4 years. An MIB-I-index was formed by counting 5000 tumor-cell nuclei in 10-15 randomly chosen high-power fields and calculating percentages of positively stained cells. CGH involved hybridization of FITC-dUTP-labeled tumor DNA with Texas-red-labeled normal DNA. Images were digitally analyzed. The MIB-1-index (range 0-2.51) was low for all tumors. No statistically significant correlation between MIB-1 index and tendency to recurrence was found. Chromosomal aberrations were detected in 2 of 17 cases. The results suggest that formation of an MIB-1 index is not helpful in assessing future clinical behavior of an ameloblastoma and that chromosomal imbalances are uncommon. (C) 2002 Elsevier Science Inc. All rights reserved.