A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Cell proliferation and chromosomal changes in human ameloblastoma
Tekijät: Jaaskelainen K, Jee KJ, Leivo I, Saloniemi I, Knuutila S, Heikinheimo K
Kustantaja: ELSEVIER SCIENCE INC
Julkaisuvuosi: 2002
Journal: Cancer Genetics and Cytogenetics
Tietokannassa oleva lehden nimi: CANCER GENETICS AND CYTOGENETICS
Lehden akronyymi: CANCER GENET CYTOGEN
Vuosikerta: 136
Numero: 1
Aloitussivu: 31
Lopetussivu: 37
Sivujen määrä: 7
ISSN: 0165-4608
DOI: https://doi.org/10.1016/S0165-4608(02)00512-5
Tiivistelmä
Cell proliferation and chromosomal imbalances, important parameters in relation to tumor progression, were studied in ameloblastoma (n=20), a benign odontogenic tumor of locally recurrent nature. Immunocytochemical staining with MIB-1 antibody and comparative genomic hybridization (CGH) were performed on formalin-fixed paraffin-embedded ameloblastomas. The mean follow-up time was 12.4 years. An MIB-I-index was formed by counting 5000 tumor-cell nuclei in 10-15 randomly chosen high-power fields and calculating percentages of positively stained cells. CGH involved hybridization of FITC-dUTP-labeled tumor DNA with Texas-red-labeled normal DNA. Images were digitally analyzed. The MIB-1-index (range 0-2.51) was low for all tumors. No statistically significant correlation between MIB-1 index and tendency to recurrence was found. Chromosomal aberrations were detected in 2 of 17 cases. The results suggest that formation of an MIB-1 index is not helpful in assessing future clinical behavior of an ameloblastoma and that chromosomal imbalances are uncommon. (C) 2002 Elsevier Science Inc. All rights reserved.
Cell proliferation and chromosomal imbalances, important parameters in relation to tumor progression, were studied in ameloblastoma (n=20), a benign odontogenic tumor of locally recurrent nature. Immunocytochemical staining with MIB-1 antibody and comparative genomic hybridization (CGH) were performed on formalin-fixed paraffin-embedded ameloblastomas. The mean follow-up time was 12.4 years. An MIB-I-index was formed by counting 5000 tumor-cell nuclei in 10-15 randomly chosen high-power fields and calculating percentages of positively stained cells. CGH involved hybridization of FITC-dUTP-labeled tumor DNA with Texas-red-labeled normal DNA. Images were digitally analyzed. The MIB-1-index (range 0-2.51) was low for all tumors. No statistically significant correlation between MIB-1 index and tendency to recurrence was found. Chromosomal aberrations were detected in 2 of 17 cases. The results suggest that formation of an MIB-1 index is not helpful in assessing future clinical behavior of an ameloblastoma and that chromosomal imbalances are uncommon. (C) 2002 Elsevier Science Inc. All rights reserved.
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