B1 Non-refereed article in a scientific journal
Protolytic and nucleophilic role of phosphoramidite method promoters
Authors: Nurminen EJ, Mattinen JK, Lonnberg H
Publication year: 2002
Journal:: Collection Symposium Series
Journal name in source: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Journal acronym: COLL SYMPOS SERIES
Volume: 5
First page : 129
Last page: 134
Number of pages: 6
ISBN: 80-86241-16-5
Abstract
The effect of promoters applied in phosphoramidite alcoholysis is based on both acid and nucleophilic catalysis. Observed Bronsted alpha values 0.6-0.9 and beta(nucl) value of 0.2 emphasise the importance of the acidic contribution. Still, highly nucleophilic azole activators of moderate acidity have shown excellent reactivity and should be considered as most promising alternatives to tetrazole as activators in oligonucleotide synthesis.
The effect of promoters applied in phosphoramidite alcoholysis is based on both acid and nucleophilic catalysis. Observed Bronsted alpha values 0.6-0.9 and beta(nucl) value of 0.2 emphasise the importance of the acidic contribution. Still, highly nucleophilic azole activators of moderate acidity have shown excellent reactivity and should be considered as most promising alternatives to tetrazole as activators in oligonucleotide synthesis.
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