B1 Vertaisarvioimaton kirjoitus tieteellisessä lehdessä
Protolytic and nucleophilic role of phosphoramidite method promoters
Tekijät: Nurminen EJ, Mattinen JK, Lonnberg H
Julkaisuvuosi: 2002
Lehti:: Collection Symposium Series
Tietokannassa oleva lehden nimi: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Lehden akronyymi: COLL SYMPOS SERIES
Vuosikerta: 5
Aloitussivu: 129
Lopetussivu: 134
Sivujen määrä: 6
ISBN: 80-86241-16-5
Tiivistelmä
The effect of promoters applied in phosphoramidite alcoholysis is based on both acid and nucleophilic catalysis. Observed Bronsted alpha values 0.6-0.9 and beta(nucl) value of 0.2 emphasise the importance of the acidic contribution. Still, highly nucleophilic azole activators of moderate acidity have shown excellent reactivity and should be considered as most promising alternatives to tetrazole as activators in oligonucleotide synthesis.
The effect of promoters applied in phosphoramidite alcoholysis is based on both acid and nucleophilic catalysis. Observed Bronsted alpha values 0.6-0.9 and beta(nucl) value of 0.2 emphasise the importance of the acidic contribution. Still, highly nucleophilic azole activators of moderate acidity have shown excellent reactivity and should be considered as most promising alternatives to tetrazole as activators in oligonucleotide synthesis.
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