A1 Refereed original research article in a scientific journal

Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling




AuthorsJakobson L, Vaahtera L, Tõldsepp K, Nuhkat M, Wang C, Wang YS, Hõrak H, Valk E, Pechter P, Sindarovska Y, Tang J, Xiao C, Xu Y, Gerst Talas U, García-Sosa AT, Kangasjärvi S, Maran U, Remm M, Roelfsema MR, Hu H, Kangasjärvi J, Loog M, Schroeder JI, Kollist H, Brosché M

PublisherPLOS

Publication year2016

JournalPLoS Biology

Journal acronymPLOS Biol

Article numbere2000322

Volume14

Issue12

Number of pages25

ISSN1545-7885

eISSN1545-7885

DOIhttps://doi.org/10.1371/journal.pbio.2000322(external)

Web address http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2000322(external)


Abstract

Plant gas exchange is regulated by guard cells that form stomatal pores.
Stomatal adjustments are crucial for plant survival; they regulate
uptake of CO2 for photosynthesis, loss of water, and entrance of air
pollutants such as ozone. We mapped ozone hypersensitivity, more open
stomata, and stomatal CO2-insensitivity phenotypes of the Arabidopsis
thaliana accession Cvi-0 to a single amino acid substitution in
MITOGEN-ACTIVATED PROTEIN (MAP) KINASE 12 (MPK12). In parallel, we
showed that stomatal CO2-insensitivity phenotypes of a mutant cis
(CO2-insensitive) were caused by a deletion of MPK12. Lack of MPK12
impaired bicarbonate-induced activation of S-type anion channels. We
demonstrated that MPK12 interacted with the protein kinase HIGH LEAF
TEMPERATURE 1 (HT1)-a central node in guard cell CO2 signaling-and that
MPK12 functions as an inhibitor of HT1. These data provide a new
function for plant MPKs as protein kinase inhibitors and suggest a
mechanism through which guard cell CO2 signaling controls plant water
management.


Downloadable publication

This is an electronic reprint of the original article.
This reprint may differ from the original in pagination and typographic detail. Please cite the original version.





Last updated on 2024-26-11 at 11:01