A1 Refereed original research article in a scientific journal
Mixotrophic culture of Chaetoceros sp and the synergistic carbon and energy metabolism
Authors: Shan Shengzhou, Wang Shanshan, Yan Xi, Chen Kang, Liang Li, Li Xiaohui, Zhou Chengxu, Yan Xiaojun, Ruan Roger, Cheng Pengfei
Publisher: Elsevier BV
Publication year: 2023
Journal: Bioresource Technology
Journal name in source: Bioresource technology
Journal acronym: Bioresour Technol
Article number: 129912
Volume: 390
ISSN: 0960-8524
eISSN: 1873-2976
DOI: https://doi.org/10.1016/j.biortech.2023.129912
Web address : https://doi.org/10.1016/j.biortech.2023.129912
Abstract
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL-1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL-1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.
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