Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis
: Jahandideh Arghavan, Virta Jenni, Li Xiang-Guo, Liljenbäck Heidi, Moisio Olli, Ponkamo Jesse, Rajala Noora, Alix Marion, Lehtonen Jukka, Mäyränpää Mikko I., Salminen Tiina A., Knuuti Juhani, Jalkanen Sirpa, Saraste Antti, Roivainen Anne
Publisher: SPRINGER
: 2023
: Journal of Nuclear Cardiology
: J NUCL CARDIOL
: 13
: 1071-3581
: 1532-6551
DOI: https://doi.org/10.1007/s12350-023-03371-8
: https://link.springer.com/article/10.1007/s12350-023-03371-8
: https://research.utu.fi/converis/portal/detail/Publication/181483610
: Correction to this article (Published: 29 May 2024): https://www.journalofnuclearcardiology.org/article/S1071-3581(24)00474-4/pdf ; DOI: 10.1016/j.nuclcard.2024.101846
Background. Vascular adhesion protein-1 (VAP-1) is an adhesion molecule and primary amine oxidase, and Gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetra-acetic acid conjugated sialic acid-binding immunoglobulin-like lectin 9 motif containing peptide ([68Ga]Ga-DOTA-Siglec-9) is a positron emission tomography (PET) tracer targeting VAP-1. We evaluated the feasibility of PET imaging with [68Ga]Ga-DOTA-Siglec-9 for the detection of myocardial lesions in rats with autoimmune myocarditis.
Methods. Rats (n = 9) were immunized twice with porcine cardiac myosin in complete Freund's adjuvant. Control rats (n = 6) were injected with Freund's adjuvant alone. On day 21, in vivo PET/computed tomography (CT) imaging with [68Ga]Ga-DOTA-Siglec-9 was performed, followed by ex vivo autoradiography, histology, and immunohistochemistry of tissue sections. In addition, myocardial samples from three patients with cardiac sarcoidosis were studied.
Results. [68Ga]Ga-DOTA-Siglec-9 PET/CT images of immunized rats showed higher uptake in myocardial lesions than in myocardium outside lesions (SUVmean,0.5 ± 0.1 vs 0.3 ± 0.1; P = .003) or control rats (SUVmean,0.2 ± 0.03; P < .0001), which was confirmed by ex vivo autoradiography of tissue sections. Immunohistochemistry showed VAP-1-positive staining in lesions of rats with myocarditis and in patients with cardiac sarcoidosis.Conclusion. VAP-1-targeted [Ga-68]Ga-DOTA-Siglec-9 PET is a potential novel technique for the detection of myocardial lesions.
