A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm
Tekijät: Nieminen H, Nummela P, Satomaa T, Heiskanen A, Hiltunen JO, Kaprio T, Seppänen H, Hagström J, Mustonen H, Ristimäki A, Haglund C
Kustantaja: NATURE PORTFOLIO
Julkaisuvuosi: 2023
Journal: Scientific Reports
Tietokannassa oleva lehden nimi: SCIENTIFIC REPORTS
Lehden akronyymi: SCI REP-UK
Vuosikerta: 13
Numero: 1
Sivujen määrä: 12
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-023-39220-4
Verkko-osoite: https://doi.org/10.1038/s41598-023-39220-4
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/180901927
Intraductal papillary mucinous neoplasms (IPMNs), often found incidentally, are potentially malignant cystic tumors of the pancreas. Due to the precancerous nature, IPMNs lacking malignant features should be kept on surveillance. The follow-up relies on magnetic resonance imaging, which has a limited accuracy to define the high-risk patients. New diagnostic methods are thus needed to recognize IPMNs with malignant potential. Here, aberrantly expressed glycans constitute a promising new area of research. We compared the N-glycan profiles of non-invasive IPMN tissues (n = 10) and invasive IPMN tissues (n = 10) to those of non-neoplastic pancreatic controls (n = 5) by matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. Both IPMN subgroups showed increased abundance of neutral composition H4N4 and decrease in H3N5F1, increase in sialylation, and decrease in sulfation, as compared to the controls. Furthermore, invasive IPMN showed an increase in terminal N-acetylhexosamine containing structure H4N5, and increase in acidic complex-type glycans, but decrease in their complex fucosylation and sulfation, as compared to the controls. In conclusion, the N-glycan profiles differed between healthy pancreatic tissue and non-invasive and invasive IPMNs. The unique glycans expressed in invasive IPMNs may offer interesting new options for diagnostics.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
