Other publication

Liquid Crystalline Behavior of Bifunctional PhageBiosensor in Biomedical Detection




AuthorsJuusti Vilhelmiina, Kulpakko Janne, Cudjoe Elizabeth, Amoah Linda E., Pimenoff Ville, Hänninen Pekka

Publication year2023

JournalEuropean Biophysics Journal

Volume52

IssueSuppl. 1

First page S188

Last pageS188

DOIhttps://doi.org/10.1007/s00249-023-01668-7(external)

Web address https://doi.org/10.1007/s00249-023-01668-7(external)


Abstract

Biomedical applications of phages have been extensively researched during the past decade. The combination of in vitro selected phages and their intrinsic exhibition of liquid crystalline (LC) behavior can be utilized in novel biosensors. We have developed bifunctional phage-biosensors for optical detection of the target analytes with time-resolved fluorescence (TRF) and absorbance. Phages offer a tempting alternative to develop new biomedical detection methods. The Green Fluorescent Protein (GFP) was used as a model analyte due to its well known optical properties. The phage-derived GFP detection was measured and analyzed in relation to modulation in TRF signal and color formation with absorbance. The LC behavior during the GFP detection was studied by measuring refractive indexes and biosensor performance in lyotropic and thermotropic conditions. Furthermore, we developed a biosensor to detect Plasmodium falciparum in the saliva of symptomatic and asymptomatic malaria patients. We found that the optical properties of the phagebiosensor system changed accordingly with the GFP concentration. The results with TRF and absorbance reflected the changes in LC behavior. The biosensor reached the limit of detection of 0.24 µg/mL with GFP. Respectively, the accuracy of 90% in detection of Plasmodium falciparum infection from saliva was reached in 15 minutes. Our results provide valuable information about the optical changes of the phage-biosensor in relation to the presence of analytes. The LC behavior of the biosensor enables quantitative analyte detection as well as disease detection from saliva. Overall, we suggest that the method is suitable for rapid screening of biomedical matrices.



Last updated on 2024-26-11 at 11:13