A1 Refereed original research article in a scientific journal
Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics
Authors: Dhondt Bert, Pinheiro Cláudio, Geeurickx Edward, Tulkens Joeri, Vergauwen Glenn, Van Der Pol Edwin, Nieuwland Rienk, Decock Anneleen, Miinalainen Ilkka, Rappu Pekka, Schroth Gary, Kuersten Scott, Vandesompele Jo, Mestdagh Pieter, Lumen Nicolaas, De Wever Olivier, Hendrix An
Publisher: Wiley
Publication year: 2023
Journal: Journal of Extracellular Vesicles
Journal name in source: JOURNAL OF EXTRACELLULAR VESICLES
Journal acronym: J EXTRACELL VESICLES
Article number: e12315
Volume: 12
Issue: 5
Number of pages: 28
eISSN: 2001-3078
DOI: https://doi.org/10.1002/jev2.12315
Web address : https://doi.org/10.1002/jev2.12315
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/179760315
The analysis of extracellular vesicles (EV) in blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently assess EV-associated biomarkers, but the impact of pre-analytics on EV characteristics in blood samples remains minimally explored. We present the results from the first large-scale EV Blood Benchmarking (EVBB) study in which we systematically compared 11 blood collection tubes (BCT; six preservation and five non-preservation) and three blood processing intervals (BPI; 1, 8 and 72 h) on defined performance metrics (n = 9). The EVBB study identifies a significant impact of multiple BCT and BPI on a diverse set of metrics reflecting blood sample quality, ex-vivo generation of blood-cell derived EV, EV recovery and EV-associated molecular signatures. The results assist the informed selection of the optimal BCT and BPI for EV analysis. The proposed metrics serve as a framework to guide future research on pre-analytics and further support methodological standardization of EV studies.
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