A1 Refereed original research article in a scientific journal
Alternative Copper-Based Single-Atom Nanozyme with Superior Multienzyme Activities and NIR-II Responsiveness to Fight against Deep Tissue Infections
Authors: Bai Jiaxiang, Feng Yonghai, Li Wenming, Cheng Zerui, Rosenholm Jessica M, Yang Huilin, Pan Guoqing, Zhang Hongbo, Geng Dechun
Publisher: American Association for the Advancement of Science
Publication year: 2023
Journal: Research
Journal name in source: Research (Washington, D.C.)
Article number: 0031
Volume: 6
ISSN: 2639-5274
eISSN: 2639-5274
DOI: https://doi.org/10.34133/research.0031(external)
Web address : https://spj.science.org/doi/10.34133/research.0031(external)
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/179519542(external)
Nanozymes are considered to represent a new era of antibacterial agents, while their antibacterial efficiency is limited by the increasing tissue depth of infection. To address this issue, here, we report a copper and silk fibroin (Cu-SF) complex strategy to synthesize alternative copper single-atom nanozymes (SAzymes) with atomically dispersed copper sites anchored on ultrathin 2D porous N-doped carbon nanosheets (CuN x -CNS) and tunable N coordination numbers in the CuN x sites (x = 2 or 4). The CuN x -CNS SAzymes inherently possess triple peroxidase (POD)-, catalase (CAT)-, and oxidase (OXD)-like activities, facilitating the conversion of H2O2 and O2 into reactive oxygen species (ROS) through parallel POD- and OXD-like or cascaded CAT- and OXD-like reactions. Compared to CuN2-CNS, tailoring the N coordination number from 2 to 4 endows the SAzyme (CuN4-CNS) with higher multienzyme activities due to its superior electron structure and lower energy barrier. Meanwhile, CuN x -CNS display strong absorption in the second near-infrared (NIR-II) biowindow with deeper tissue penetration, offering NIR-II-responsive enhanced ROS generation and photothermal treatment in deep tissues. The in vitro and in vivo results demonstrate that the optimal CuN4-CNS can effectively inhibit multidrug-resistant bacteria and eliminate stubborn biofilms, thus exhibiting high therapeutic efficacy in both superficial skin wound and deep implant-related biofilm infections.
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