A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Monocarbonyl Curcumin Analogues as Potent Inhibitors against Human Glutathione Transferase P1-1
Tekijät: Pantiora Panagiota, Furlan Veronika, Matiadis Dimitris, Mavroidi Barbara, Perperopoulou Fereniki, Papageorgiou Anastassios C, Sagnou Marina, Bren Urban, Pelecanou Maria, Labrou Nikolaos E
Kustantaja: MDPI
Julkaisuvuosi: 2023
Journal: Antioxidants
Tietokannassa oleva lehden nimi: Antioxidants (Basel, Switzerland)
Lehden akronyymi: Antioxidants (Basel)
Vuosikerta: 12
Numero: 1
ISSN: 2076-3921
eISSN: 2076-3921
DOI: https://doi.org/10.3390/antiox12010063
Verkko-osoite: https://www.doi.org/10.3390/antiox12010063
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/178536174
The isoenzyme of human glutathione transferase P1-1 (hGSTP1-1) is involved in multi-drug resistance (MDR) mechanisms in numerous cancer cell lines. In the present study, the inhibition potency of two curcuminoids and eleven monocarbonyl curcumin analogues against hGSTP1-1 was investigated. Demethoxycurcumin (Curcumin II) and three of the monocarbonyl curcumin analogues exhibited the highest inhibitory activity towards hGSTP1-1 with IC50 values ranging between 5.45 ± 1.08 and 37.72 ± 1.02 μM. Kinetic inhibition studies of the most potent inhibitors demonstrated that they function as non-competitive/mixed-type inhibitors. These compounds were also evaluated for their toxicity against the prostate cancer cells DU-145. Interestingly, the strongest hGSTP1-1 inhibitor, (DM96), exhibited the highest cytotoxicity with an IC50 of 8.60 ± 1.07 μΜ, while the IC50 values of the rest of the compounds ranged between 44.59-48.52 μΜ. Structural analysis employing molecular docking, molecular dynamics (MD) simulations, and binding-free-energy calculations was performed to study the four most potent curcumin analogues as hGSTP1-1 inhibitors. According to the obtained computational results, DM96 exhibited the lowest binding free energy, which is in agreement with the experimental data. All studied curcumin analogues were found to form hydrophobic interactions with the residue Gln52, as well as hydrogen bonds with the nearby residues Gln65 and Asn67. Additional hydrophobic interactions with the residues Phe9 and Val36 as well as π-π stacking interaction with Phe9 contributed to the superior inhibitory activity of DM96. The van der Waals component through shape complementarity was found to play the most important role in DM96-inhibitory activity. Overall, our results revealed that the monocarbonyl curcumin derivative DM96 acts as a strong hGSTP1-1 inhibitor, exerts high prostate cancer cell cytotoxicity, and may, therefore, be exploited for the suppression and chemosensitization of cancer cells. This study provides new insights into the development of safe and effective GST-targeted cancer chemosensitizers.
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