A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Unraveling the malate biosynthesis during development of Torreya grandis nuts
Tekijät: Yan Jingwei, Chen Weijie, Zeng Hao, Cheng Hao, Suo Jinwei, Yu Chenliang, Yang Baoru, Lou Heqiang, Song Lili, Wu Jiasheng
Kustantaja: Elsevier BV
Julkaisuvuosi: 2022
Journal: Current Research in Food Science
Tietokannassa oleva lehden nimi: Current research in food science
Lehden akronyymi: Curr Res Food Sci
Vuosikerta: 5
Aloitussivu: 2309
Lopetussivu: 2315
ISSN: 2665-9271
eISSN: 2665-9271
DOI: https://doi.org/10.1016/j.crfs.2022.11.017
Verkko-osoite: https://doi.org/10.1016/j.crfs.2022.11.017
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/177720928
Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The kernels of T. grandis have rich content of organic acids, and malate is the predominant organic acid in T. grandis kernels. However, the contents, biosynthesis/metabolism pathway and transcriptional regulation of malate in developing T. grandis kernels remain completely unknown. Here, the organic acid composition in developing T. grandis kernels was first analyzed. The results showed that the content of malate was increased during the maturation T. grandis kernels. A malate synthase (TgMLS) gene might be involved in the accumulation of malate based on transcriptome data, gene expression and enzyme activity analysis. Transient expression of TgMLS in tobacco resulted in the high malate synthase activity and malate content. Furthermore, a basic helix-loop-helix transcription factor (bHLH), TgbHLH87 was identified to positively regulate the TgMLS expression via directly binding the TgMLS promoter. Our finding contributes to mechanism underlying malate accumulation in T. grandis kernels.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
